Pipeline for illumination correction of images for high‐throughput microscopy
نویسندگان
چکیده
منابع مشابه
Pipeline for illumination correction of images for high-throughput microscopy
The presence of systematic noise in images in high-throughput microscopy experiments can significantly impact the accuracy of downstream results. Among the most common sources of systematic noise is non-homogeneous illumination across the image field. This often adds an unacceptable level of noise, obscures true quantitative differences and precludes biological experiments that rely on accurate...
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BACKGROUND Many digital microscopy images suffer from poor illumination at the peripheries (vignetting), often attributable to factors related to the light path between the camera and the microscope. A commonly used method for correcting this has been to use the illumination of an empty field as a correction filter (white shading correction). AIMS To develop an alternative shading correction ...
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With the growth of technology, supervising systems are increasingly replacing humans in military, transportation, medical, spatial, and other industries. Among these systems are machine vision systems which are based on image processing and analysis. One of the important tasks of image processing is classification of images into desirable categories for the identification of objects or their sp...
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RF-Inhomogeneity Correction (aka bias) artifact is an important research field in Magnetic Resonance Imaging (MRI). Bias corrupts MR images altering their illumination even though they are acquired with the most recent scanners. Homomorphic Unsharp Masking (HUM) is a filtering technique aimed at correcting illumination inhomogeneity, but it produces a halo around the edges as a side effect. In ...
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Recently, a light sheet-based technique called objective-coupled planar illumination (OCPI) microscopy [Holekamp et al., Neuron 57, 661 (2008)] was shown to permit low-phototoxicity, high-speed, three-dimensional fluorescence imaging of extended tissue samples. Here, we introduce two major improvements in OCPI microscopy. First, we miniaturize the objective coupler by using a uniaxial gradient-...
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ژورنال
عنوان ژورنال: Journal of Microscopy
سال: 2014
ISSN: 0022-2720,1365-2818
DOI: 10.1111/jmi.12178